Klenow fragment

DNA primase activity was assayed with E coli . DNA polymerase ⅰ Klenow fragment extended primers . The specific activity of the enzyme was 8 753 U / mg protein .

用大肠杆菌大片段DNA多聚酶Ⅰ延长引物法检测DNA引物酶比活性为8753U／mg，酶总获得为22.1%。

In situ detection of neuron DNA strand early breaks using the Klenow fragment of DNA polymerase - ⅰ

DNA聚合酶-ⅠKlenow片段原位检测缺血性神经元的DNA早期断裂

Results and conclusion : Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system .

结果与结论：Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果，且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。

However , the essential content of law has its own form which is the inner structural character . coli Klenow fragment random primer DNA labeling system served as control .

以大肠杆菌Klenow片断随机引物延伸标记法作为对照。

Methods Neurons number and morphologic change were observed through Nissl staining method , and DNA strand breaks were in situ detected by DNA polymerase ⅰ Klenow fragment mediated nick end labeling method .

方法使用尼氏染色方法观察再灌注早期损伤区域神经元数量和形态的变化，使用DNA聚合酶-Klenow片段原位检测DNA的断裂损伤。

Methods : The circled TA plasmid was digested and linearized with restriction endonuclease . The ends of the plasmid were repaired and made blunt with Klenow Fragment . A single T was added respectively to the 5 ′ ends of the vector with Taq DNA polymerase .

方法：将环化质粒以限制性内切酶消化成线性后，以Klenow大片段将末端补平，再用taqDNA聚合酶在线性载体的两个5′末端各加上一个非配对的T。

Two kinds of mecA and femA probes of staphylococcus aureus were immobilized on the gold electrode surface of QOGS array . Klenow large fragment was added to extend the strand in room temperature after the probes were hybridized with corresponding target DNA . 4 .

基因传感器阵列表面分别固定上金黄色葡萄球菌mecA及femA两种探针片段，待杂交上相应的靶序列片段后，加入Klenow酶在室温下进行链延伸反应。